[PDF] Molecular Interaction Fields Gabriele Cruciani, Raimund Mannhold, Hugo Kubinyi, Gerd Folkers

Thanks to the vast progress made in the investigation of biomolecules using NMR during the later 1990s, NMR spectroscopy offers a universal tool for examining the binding of an active substance to its target protein. Its use also benefits the development of drugs in particular. Content: Part I: Basic techniques — Modern methods for the expression of proteins in isotopically enriched form / Heiko Patzelt … [et al.] — Structure calculation using automated techniques / Peter Gu?ntert — Achieving better sensitivity, less noise and fewer artifacts in NMR spectra / Detlef Moskau and Oliver Zerbe — Part II: NMR of biomolecules — NMR strategies for protein assignments / Volker Do?tsch — NMR of membrane-associated peptides and proteins / Reto Bader, Mirjam Lerch, and Oliver Zerbe — NMR of nucleic acids / Radovan Fiala and Vladimi?r Sklena?r? — Part III: Modern spectroscopic techniques — Methods for the measurement of angle restraints from scalar, dipolar couplings and from cross-correlated relaxation : application to biomacromolecules / Christian Griesinger — Orientational restraints / Eva de Alba and Nico Tjandra — Scalar couplings across hydrogen bonds / Andrew J. Dingley … [et al.] — TROSY : transverse relaxation-optimized spectroscopy / Roland Riek — MAS solid-state NMR of isotopically enriched biological samples / Philip T.F. Williamson, Matthias Ernst, and Beat H. Meier — Determination of protein dynamics using [superscript 15]N relaxation measurements / David Fushman — Part IV: Tools for investigation of drug-receptor complexes and for ligand screening — The determination of equilibrium dissociation constants of protein-ligand complexes by NMR / Gordon C.K. Roberts — Experiments in NMR-based screening / Carla Marchioro … [et al.] — The use of spin labels in NMR-supported lead finding and optimization / Wolfgang Jahnke — NMR of weakly binding ligands / Marcel J.J. Blommers and Simon Ru?disser — Isotope filter and editing techniques / Gerd Gemmecker — Part V: Strategies for drug development using NMR — Strategies for NMR screening and library design / Christopher A. Lepre — Strategies for hit finding using NMR / Werner Klaus and Hans Senn. Strategies for drug discovery using NMR / Marcel J.J. Blommers, Andreas Flo?rsheimer, and Wolfgang Jahnke — NMR-based drug design : approaches for very large proteins / Maurizio Pellecchia … [et al.]. Abstract: Thanks to the vast progress made in the investigation of biomolecules using NMR during the later 1990s, NMR spectroscopy offers a universal tool for examining the binding of an active substance to its target protein. Its use also benefits the development of drugs in particular